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ROS Induction Targets Persister Cancer Cells with Low Metabolic Activity in NRAS-Mutated Melanoma.
- Source :
-
Cancer research [Cancer Res] 2023 Apr 04; Vol. 83 (7), pp. 1128-1146. - Publication Year :
- 2023
-
Abstract
- Clinical management of melanomas with NRAS mutations is challenging. Targeting MAPK signaling is only beneficial to a small subset of patients due to resistance that arises through genetic, transcriptional, and metabolic adaptation. Identification of targetable vulnerabilities in NRAS-mutated melanoma could help improve patient treatment. Here, we used multiomics analyses to reveal that NRAS-mutated melanoma cells adopt a mesenchymal phenotype with a quiescent metabolic program to resist cellular stress induced by MEK inhibition. The metabolic alterations elevated baseline reactive oxygen species (ROS) levels, leading these cells to become highly sensitive to ROS induction. In vivo xenograft experiments and single-cell RNA sequencing demonstrated that intratumor heterogeneity necessitates the combination of a ROS inducer and a MEK inhibitor to inhibit both tumor growth and metastasis. Ex vivo pharmacoscopy of 62 human metastatic melanomas confirmed that MEK inhibitor-resistant tumors significantly benefited from the combination therapy. Finally, oxidative stress response and translational suppression corresponded with ROS-inducer sensitivity in 486 cancer cell lines, independent of cancer type. These findings link transcriptional plasticity to a metabolic phenotype that can be inhibited by ROS inducers in melanoma and other cancers.<br />Significance: Metabolic reprogramming in drug-resistant NRAS-mutated melanoma cells confers sensitivity to ROS induction, which suppresses tumor growth and metastasis in combination with MAPK pathway inhibitors.<br /> (©2023 American Association for Cancer Research.)
- Subjects :
- Humans
Reactive Oxygen Species
Proto-Oncogene Proteins B-raf genetics
Protein Kinase Inhibitors therapeutic use
Mitogen-Activated Protein Kinase Kinases genetics
Cell Line, Tumor
Mutation
Membrane Proteins genetics
GTP Phosphohydrolases genetics
Melanoma drug therapy
Melanoma genetics
Melanoma pathology
Skin Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1538-7445
- Volume :
- 83
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 36946761
- Full Text :
- https://doi.org/10.1158/0008-5472.CAN-22-1826