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Carrageenan from Gigartina skottsbergii : A Novel Molecular Probe to Detect SARS-CoV-2.
- Source :
-
Biosensors [Biosensors (Basel)] 2023 Mar 13; Vol. 13 (3). Date of Electronic Publication: 2023 Mar 13. - Publication Year :
- 2023
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Abstract
- The COVID-19 pandemic has caused an unprecedented health and economic crisis, highlighting the importance of developing new molecular tools to monitor and detect SARS-CoV-2. Hence, this study proposed to employ the carrageenan extracted from Gigartina skottsbergii algae as a probe for SARS-CoV-2 virus binding capacity and potential use in molecular methods. G. skottsbergii specimens were collected in the Chilean subantarctic ecoregion, and the carrageenan was extracted -using a modified version of Webber's method-, characterized, and quantified. After 24 h of incubation with an inactivated viral suspension, the carrageenan's capacity to bind SARS-CoV-2 was tested. The probe-bound viral RNA was quantified using the reverse transcription and reverse transcription loop-mediated isothermal amplification (RT-LAMP) methods. Our findings showed that carrageenan extraction from seaweed has a similar spectrum to commercial carrageenan, achieving an excellent proportion of binding to SARS-CoV-2, with a yield of 8.3%. Viral RNA was also detected in the RT-LAMP assay. This study shows, for the first time, the binding capacity of carrageenan extracted from G. skottsbergii , which proved to be a low-cost and highly efficient method of binding to SARS-CoV-2 viral particles.
Details
- Language :
- English
- ISSN :
- 2079-6374
- Volume :
- 13
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biosensors
- Publication Type :
- Academic Journal
- Accession number :
- 36979590
- Full Text :
- https://doi.org/10.3390/bios13030378