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3'-End Processing of Eukaryotic mRNA: Machinery, Regulation, and Impact on Gene Expression.
- Source :
-
Annual review of biochemistry [Annu Rev Biochem] 2023 Jun 20; Vol. 92, pp. 199-225. Date of Electronic Publication: 2023 Mar 31. - Publication Year :
- 2023
-
Abstract
- Formation of the 3' end of a eukaryotic mRNA is a key step in the production of a mature transcript. This process is mediated by a number of protein factors that cleave the pre-mRNA, add a poly(A) tail, and regulate transcription by protein dephosphorylation. Cleavage and polyadenylation specificity factor (CPSF) in humans, or cleavage and polyadenylation factor (CPF) in yeast, coordinates these enzymatic activities with each other, with RNA recognition, and with transcription. The site of pre-mRNA cleavage can strongly influence the translation, stability, and localization of the mRNA. Hence, cleavage site selection is highly regulated. The length of the poly(A) tail is also controlled to ensure that every transcript has a similar tail when it is exported from the nucleus. In this review, we summarize new mechanistic insights into mRNA 3'-end processing obtained through structural studies and biochemical reconstitution and outline outstanding questions in the field.
- Subjects :
- Humans
RNA, Messenger genetics
RNA, Messenger metabolism
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Gene Expression
RNA Precursors genetics
RNA Precursors metabolism
mRNA Cleavage and Polyadenylation Factors genetics
mRNA Cleavage and Polyadenylation Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1545-4509
- Volume :
- 92
- Database :
- MEDLINE
- Journal :
- Annual review of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 37001138
- Full Text :
- https://doi.org/10.1146/annurev-biochem-052521-012445