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Enhanced removal of warfare agent tri-nitro-toluene by a Methylophaga-dominated microbiome.

Authors :
Kundu K
Van Landuyt J
Mattelin V
Martin B
Neyts M
Parmentier K
Boon N
Source :
Marine pollution bulletin [Mar Pollut Bull] 2023 May; Vol. 190, pp. 114866. Date of Electronic Publication: 2023 Mar 29.
Publication Year :
2023

Abstract

Historical exposure of the marine environment to 2,4,6-trinitrotoluene (TNT) happened due to the dumping of left-over munitions. Despite significant research on TNT decontamination, the potential of marine microbiome for TNT degradation remains only little explored. In this study, TNT degradation experiments were conducted with sediment located near the World War I munition dumpsite - Paardenmarkt in the Belgian part of North Sea. A slow removal was observed using TNT as sole source of C and N, which could be enhanced by adding methanol. Degradation was reflected in nitro-reduced metabolites and microbial growth. 16S Illumina sequencing analysis revealed several enriched genera that used TNT as a sole source of C and N - Colwellia, Thalossospira, and Methylophaga. Addition of methanol resulted in increased abundance of Methylophaga, which corresponded to the rapid removal of TNT. Methanol enhanced the degradation by providing additional energy and establishing syntrophic association between methanol-utilizing and TNT-utilizing bacteria.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2023. Published by Elsevier Ltd.)

Details

Language :
English
ISSN :
1879-3363
Volume :
190
Database :
MEDLINE
Journal :
Marine pollution bulletin
Publication Type :
Academic Journal
Accession number :
37001405
Full Text :
https://doi.org/10.1016/j.marpolbul.2023.114866