Synthesis of Peptides and Proteins with Site-Specific Glutamate Arginylation.

Solid-phase peptide synthesis and protein semi-synthesis are powerful methods for site-specific modification of peptides and proteins. We describe protocols using these techniques for the syntheses of peptides and proteins bearing glutamate arginylation (E ^Arg ) at specific sites. These methods overcome challenges posed by enzymatic arginylation methods and allow for a comprehensive study of the effects of E ^Arg on protein folding and interactions. Potential applications include biophysical analyses, cell-based microscopic studies, and profiling of E ^Arg levels and interactomes in human tissue samples.
(© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)