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Preparation of MnO 2 @poly-(DMAEMA-co-IA)-conjugated methotrexate nano-complex for MRI and radiotherapy of breast cancer application.
- Source :
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Magma (New York, N.Y.) [MAGMA] 2023 Oct; Vol. 36 (5), pp. 779-795. Date of Electronic Publication: 2023 Apr 19. - Publication Year :
- 2023
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Abstract
- Objective: A novel efficient pH-sensitive targeted magnetic resonance imaging (MRI) contrast agent and innovative radio-sensitizing system were synthesized based on MnO <subscript>2</subscript> NPs coated with biocompatible poly-dimethyl-amino-ethyl methacrylate-Co-itaconic acid, (DMAEMA-Co-IA) and targeted with methotrexate (MTX).<br />Materials and Methods: The as-established NPs were fully characterized and evaluated for MRI signal enhancement, relaxivity, in vitro cell targeting, cell toxicity, blood compatibility, and radiotherapy (RT) efficacy.<br />Results: The targeted NPs MnO <subscript>2</subscript> @Poly(DMAEMA-Co-IA) and MTX-loaded NPs inhibited MCF-7 cell viability more effectively than free MTX after 24 and 48 h, respectively, with no noticeable toxicity. Additionally, the insignificant hemolytic activity demonstrated their proper hemo-compatibility. T <subscript>1</subscript> -weighted magnetic resonance imaging was used to distinguish the differential uptake of the produced MnO <subscript>2</subscript> @Poly(DMAEMA-Co-IA)-MTX NPs in malignant cells compared to normal ones in the presence of high and low MTX receptor cells (MCF-7 and MCF-10A, respectively). In MRI, the produced theranostic NPs displayed pH-responsive contrast enhancement. As shown by in vitro assays, treatment of cells with MnO <subscript>2</subscript> @Poly(DMAEMA-Co-IA)-MTX NPs prior to radiotherapy in hypoxic conditions significantly enhanced therapeutic efficacy.<br />Conclusion: We draw the conclusion that using MnO <subscript>2</subscript> @Poly(DMAEMA-Co-IA)-MTX NPs in MR imaging and combination radiotherapy may be a successful method for imaging and radiation therapy of hypoxia cells.<br /> (© 2023. The Author(s), under exclusive licence to European Society for Magnetic Resonance in Medicine and Biology (ESMRMB).)
Details
- Language :
- English
- ISSN :
- 1352-8661
- Volume :
- 36
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Magma (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 37074514
- Full Text :
- https://doi.org/10.1007/s10334-023-01091-1