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The SsWRKY1 transcription factor of Saccharum spontaneum enhances drought tolerance in transgenic Arabidopsis thaliana and interacts with 21 potential proteins to regulate drought tolerance in S. spontaneum.
- Source :
-
Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2023 Jun; Vol. 199, pp. 107706. Date of Electronic Publication: 2023 Apr 15. - Publication Year :
- 2023
-
Abstract
- In this study, we characterized a WRKY family member gene, SsWRKY1, which is located in the nucleus and contains multiple stress-related cis-acting elements. In addition, constructed SsWRKY1-overexpressing Arabidopsis thaliana had higher antioxidant enzyme activity and proline content under drought stress conditions, with lower malondialdehyde content and reactive oxygen species (ROS) accumulation, and the expression levels of six stress-related genes were significantly upregulated. This indicates that the overexpression of SsWRKY1 in Arabidopsis thaliana improves resistance to drought stress. SsWRKY1 does not have transcriptional autoactivation activity in yeast cells. The yeast two-hybrid (Y2H) system and the S. spontaneum cDNA library were used to screen 21 potential proteins that interact with SsWRKY1, and the interaction between SsWRKY1 and ATAF2 was verified by GST pull-down assay. In summary, our results indicate that SsWRKY1 plays an important role in the response to drought stress and provide initial insights into the molecular mechanism of SsWRKY1 in response to drought stress.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2023 Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Transcription Factors genetics
Transcription Factors metabolism
Drought Resistance
Plant Proteins genetics
Plant Proteins metabolism
Saccharomyces cerevisiae metabolism
Plants, Genetically Modified genetics
Gene Expression Regulation, Plant
Droughts
Antioxidants metabolism
Stress, Physiological genetics
Arabidopsis genetics
Arabidopsis metabolism
Arabidopsis Proteins genetics
Arabidopsis Proteins metabolism
Saccharum genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2690
- Volume :
- 199
- Database :
- MEDLINE
- Journal :
- Plant physiology and biochemistry : PPB
- Publication Type :
- Academic Journal
- Accession number :
- 37119548
- Full Text :
- https://doi.org/10.1016/j.plaphy.2023.107706