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eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content.
- Source :
-
The EMBO journal [EMBO J] 2023 Jun 15; Vol. 42 (12), pp. e112362. Date of Electronic Publication: 2023 May 08. - Publication Year :
- 2023
-
Abstract
- eIF3, whose subunits are frequently overexpressed in cancer, regulates mRNA translation from initiation to termination, but mRNA-selective functions of individual subunits remain poorly defined. Using multiomic profiling upon acute depletion of eIF3 subunits, we observed that while eIF3a, b, e, and f markedly differed in their impact on eIF3 holo-complex formation and translation, they were each required for cancer cell proliferation and tumor growth. Remarkably, eIF3k showed the opposite pattern with depletion promoting global translation, cell proliferation, tumor growth, and stress resistance through repressing the synthesis of ribosomal proteins, especially RPS15A. Whereas ectopic expression of RPS15A mimicked the anabolic effects of eIF3k depletion, disruption of eIF3 binding to the 5'-UTR of RSP15A mRNA negated them. eIF3k and eIF3l are selectively downregulated in response to endoplasmic reticulum and oxidative stress. Supported by mathematical modeling, our data uncover eIF3k-l as a mRNA-specific module which, through controlling RPS15A translation, serves as a rheostat of ribosome content, possibly to secure spare translational capacity that can be mobilized during stress.<br /> (© 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license.)
- Subjects :
- Humans
RNA, Messenger genetics
RNA, Messenger metabolism
Ribosomes genetics
Ribosomes metabolism
Ribosomal Proteins genetics
Ribosomal Proteins metabolism
Protein Biosynthesis
Eukaryotic Initiation Factor-3 genetics
Eukaryotic Initiation Factor-3 metabolism
Neoplasms genetics
Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2075
- Volume :
- 42
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- The EMBO journal
- Publication Type :
- Academic Journal
- Accession number :
- 37155573
- Full Text :
- https://doi.org/10.15252/embj.2022112362