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Thioredoxin1 Binding Metastasis-Associated Lung Adenocarcinoma Transcript 1 Attenuates Inflammation and Apoptosis after Intracerebral Hemorrhage.
- Source :
-
Aging and disease [Aging Dis] 2024 May 07; Vol. 15 (3), pp. 1384-1397. Date of Electronic Publication: 2024 May 07. - Publication Year :
- 2024
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Abstract
- Post-transcriptional regulation and RNA-binding proteins (RBPs) play vital roles in the occurrence of secondary injury after intracerebral hemorrhage (ICH). Therefore, we identified RBPs distinctively expressed after ICH by screening and determined thioredoxin1 (Txn1) as one of the most distinctive RBPs. We employed an ICH model and in vitro experiments to investigate the role of Txn1 in ICH. Firstly, we found that Txn1 was mainly expressed in microglia and neurons in the central nervous system, and its expression was significantly reduced in perihematomal tissue. Additionally, adeno-associated virus (AAV) carrying Txn1 was injected into the ICH rat model. Our results showed that overexpression of Txn1 reduced secondary injury and improved outcome in the ICH rat model. Moreover, to understand the therapeutic mechanism of Txn1 after ICH, we performed RNA immunoprecipitation combined with high-throughput sequencing. The results showed that Txn1 binds to inflammation- and apoptosis-related mRNAs and affects gene expression through RNA splicing and translation. Finally, RNA pull-down assays and in vitro experiments confirmed that Txn1 binds to metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), leading to reduced inflammation and apoptosis. Our study suggests that Txn1 is a potential therapeutic target for alleviating ICH-induced brain injury.
- Subjects :
- Animals
Male
Rats
Disease Models, Animal
Microglia metabolism
Microglia pathology
Rats, Sprague-Dawley
Apoptosis
Cerebral Hemorrhage pathology
Cerebral Hemorrhage genetics
Cerebral Hemorrhage metabolism
Inflammation metabolism
Inflammation genetics
Inflammation pathology
Thioredoxins genetics
Thioredoxins metabolism
RNA, Long Noncoding genetics
RNA, Long Noncoding metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2152-5250
- Volume :
- 15
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Aging and disease
- Publication Type :
- Academic Journal
- Accession number :
- 37196136
- Full Text :
- https://doi.org/10.14336/AD.2023.0507