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Enterocin LD3 from Enterococcus hirae LD3 Inhibits the Growth of Salmonella enterica subsp. enterica serovar Typhimurium ATCC 13311 in Fruit Juice.
- Source :
-
Probiotics and antimicrobial proteins [Probiotics Antimicrob Proteins] 2024 Aug; Vol. 16 (4), pp. 1205-1213. Date of Electronic Publication: 2023 Jun 17. - Publication Year :
- 2024
-
Abstract
- In order to prevent the growth of pathogens in food, bacteriocins produced by various probiotic lactic acid bacteria have been recognized as potential substitutes of chemical preservatives. In this study, enterocin LD3 was purified from the cell-free supernatant of a food isolate, Enterococcus hirae LD3 using multistep chromatography. In the fruit juice, lethal concentration (LC <subscript>50</subscript> ) of enterocin LD3 was found to be 260 µg/mL against Salmonella enterica subsp. enterica serovar Typhimurium ATCC 13311. The cells treated with enterocin LD3 were red colour indicating dead cells after propidium iodide staining, while untreated cells were found blue after staining with 4', 6-diamidino-2-phenylindole. The mechanism of cell killing was analyzed using infrared spectrum of cells treated with enterocin LD3 which was found altered in the range of 1,094.30 and 1,451.82 cm <superscript>-1</superscript> corresponding to nucleic acids and phospholipids, respectively. The morphology of target cells were severely ruptured and lysed as observed under electron microscopy. Thus, the present study suggested that enterocin LD3 showed bactericidal activity against Salm. enterica subsp. enterica serovar Typhimurium ATCC 13311 and may be applied as a bio-preservative for the safety of fruit juices.<br /> (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
- Subjects :
- Bacteriocins pharmacology
Salmonella typhimurium drug effects
Salmonella typhimurium growth & development
Bridged-Ring Compounds pharmacology
Bridged-Ring Compounds chemistry
Salmonella enterica drug effects
Salmonella enterica growth & development
Salmonella
Fruit and Vegetable Juices microbiology
Fruit and Vegetable Juices analysis
Enterococcus hirae drug effects
Enterococcus hirae growth & development
Anti-Bacterial Agents pharmacology
Anti-Bacterial Agents chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1867-1314
- Volume :
- 16
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Probiotics and antimicrobial proteins
- Publication Type :
- Academic Journal
- Accession number :
- 37330452
- Full Text :
- https://doi.org/10.1007/s12602-023-10108-z