Back to Search
Start Over
Extracellular vesicle-associated IGF2BP3 tunes Ewing sarcoma cell migration and affects PI3K/Akt pathway in neighboring cells.
- Source :
-
Cancer gene therapy [Cancer Gene Ther] 2023 Sep; Vol. 30 (9), pp. 1285-1295. Date of Electronic Publication: 2023 Jun 23. - Publication Year :
- 2023
-
Abstract
- Ewing sarcoma (EWS) is a challenging pediatric cancer characterized by vast intra-tumor heterogeneity. We evaluated the RNA-binding protein IGF2BP3, whose high expression correlates with a poor prognosis and an elevated tendency of metastases, as a possible soluble mediator of inter-cellular communication in EWS. Our data demonstrate that (i) IGF2BP3 is detected in cell supernatants, and it is released inside extracellular vesicles (EVs); (ii) EVs from IGF2BP3-positive or IGF2BP3-negative EWS cells reciprocally affect cell migration but not the proliferation of EWS recipient cells; (iii) EVs derived from IGF2BP3-silenced cells have a distinct miRNA cargo profile and inhibit the PI3K/Akt pathway in recipient cells; (iv) the 11 common differentially expressed miRNAs associated with IGF2BP3-positive and IGF2BP3-negative EVs correctly group IGF2BP3-positive and IGF2BP3-negative clinical tissue specimens. Overall, our data suggest that IGF2BP3 can participate in the modulation of phenotypic heterogeneity.<br /> (© 2023. The Author(s).)
- Subjects :
- Child
Humans
Cell Line, Tumor
Cell Movement genetics
Cell Proliferation genetics
Gene Expression Regulation, Neoplastic
Oncogene Proteins, Fusion genetics
Phosphatidylinositol 3-Kinases genetics
Proto-Oncogene Proteins c-akt metabolism
Extracellular Vesicles metabolism
Sarcoma, Ewing genetics
Sarcoma, Ewing metabolism
Sarcoma, Ewing pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1476-5500
- Volume :
- 30
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Cancer gene therapy
- Publication Type :
- Academic Journal
- Accession number :
- 37353558
- Full Text :
- https://doi.org/10.1038/s41417-023-00637-8