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Circ_0102543 suppresses hepatocellular carcinoma progression through the miR-942-5p/SGTB axis.
- Source :
-
Annals of gastroenterological surgery [Ann Gastroenterol Surg] 2023 Mar 04; Vol. 7 (4), pp. 666-677. Date of Electronic Publication: 2023 Mar 04 (Print Publication: 2023). - Publication Year :
- 2023
-
Abstract
- Introduction: Hepatocellular carcinoma (HCC) is one of the most serious cancers. Circular RNA (circRNA) has been reported to regulate the progression of HCC. Herein, the role of circ&#95;0102543 in HCC tumorigenesis was investigated.<br />Materials: The expression levels of circ&#95;0102543, microRNA-942-5p (miR-942-5p), and small glutamine rich tetratricopeptide repeat co-chaperone beta (SGTB) were detected by quantitative real-time PCR (qRT-PCR). 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide (MTT) assay, thymidine analog 5-ethynyl-2'-deoxyuridine (EDU) assay, transwell assay, and flow cytometry were conducted to explore the function of circ&#95;0102543 in HCC cells and the regulatory mechanism among circ&#95;0102543, miR-942-5p and SGTB in HCC cells. Western blot examined the related protein levels.<br />Results: The expression of circ&#95;0102543 and SGTB was decreased in HCC tissues, while the expression of miR-942-5p was increased. Circ&#95;0102543 acted as a sponge for miR-942-5p, and SGTB was the target of miR-942-5p. Circ&#95;0102543 up-regulation hindered tumor growth in vivo. In vitro experiments showed that overexpression of circ&#95;0102543 significantly repressed the malignant behaviors of HCC cells, while co-transfection of miR-942-5p partially attenuated these effects mediated by circ&#95;0102543. In addition, SGTB knockdown increased the proliferation, migration, and invasion of HCC cells inhibited by miR-942-5p inhibitor. Mechanically, circ&#95;0102543 regulated SGTB expression in HCC cells by sponging miR-942-5p.<br />Conclusion: Overexpression of circ&#95;0102543 suppressed proliferation, migration, and invasion of HCC cells by regulating the miR-942-5p/SGTB axis, suggesting that circ&#95;0102543/miR-942-5p/SGTB axis may be a potential therapeutic target for HCC.<br />Competing Interests: The authors declare that they have no competing interests.<br /> (© 2023 The Authors. Annals of Gastroenterological Surgery published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Gastroenterological Surgery.)
Details
- Language :
- English
- ISSN :
- 2475-0328
- Volume :
- 7
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Annals of gastroenterological surgery
- Publication Type :
- Academic Journal
- Accession number :
- 37416745
- Full Text :
- https://doi.org/10.1002/ags3.12665