Quantitation of DNA Using the Applied Biosystems Quantifiler ® Trio DNA Quantification Kit.

Following the isolation of deoxyribonucleic acid (DNA) from biological samples, the quantitation of amplifiable human DNA is a critical next step in the process of DNA analysis. The Quantifiler ^® Trio kit provides a simple way to accurately estimate the quantity of human and male DNA with concentrations as low as 5 pg/μL or less. Not only can the Quantifiler ^® Trio kit determine the quantity of human DNA present, but it can also give an indication of the quality of the sample, which is essential information to have in the decision-making process regarding any downstream testing being done. In this chapter, we describe how to prepare and process quantitation reactions using the Quantifiler ^® Trio kit. We also provide basic information on how to interpret the results.
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