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Efficacy of pterostilbene suppression on Aspergillus flavus growth, aflatoxin B 1 biosynthesis and potential mechanisms.

Authors :
Hu YM
Wang YR
Zhao WB
Ding YY
Wu ZR
Wang GH
Deng P
Zhang SY
An JX
Zhang ZJ
Luo XF
Liu YQ
Source :
International journal of food microbiology [Int J Food Microbiol] 2023 Nov 02; Vol. 404, pp. 110318. Date of Electronic Publication: 2023 Jul 10.
Publication Year :
2023

Abstract

Aspergillus flavus, a widespread saprotrophic filamentous fungus, could colonize agricultural crops with aflatoxin contamination, which endangers food security and the agricultural economy. A safe, effective and environmentally friendly fungicide is urgently needed. Pterostilbene, a natural phytoalexin originated from Pterocarpus indicus Willd., Vaccinium spp. and Vitis vinifera L., has been reported to possess excellent antimicrobial activity. More importantly, it is quite safe and healthy. In our screening tests of plant polyphenols for the inhibition of A. flavus, we found that pterostilbene evidently inhibited mycelial growth of Aspergillus flavus (EC <subscript>50</subscript>  = 15.94 μg/mL) and the inhibitory effect was better than that of natamycin (EC <subscript>50</subscript>  = 22.01 μg/mL), which is a natural product widely used in food preservation. Therefore, we provided insights into the efficacy of pterostilbene suppression on A. flavus growth, aflatoxin B <subscript>1</subscript> biosynthesis and its potential mechanisms against A. flavus in the present study. Here, pterostilbene at concentrations of 250 and 500 μg/mL could effectively inhibit the infection of A. flavus on peanuts. And the biosynthesis of the secondary metabolite aflatoxin B <subscript>1</subscript> was also inhibited. The antifungal effects of pterostilbene are exerted by inducing a large amount of intracellular reactive oxygen species production to bring the cells into a state of oxidative stress, damaging cellular biomolecules such as DNA, proteins and lipids and destroying the integrity of the cell membrane. Taken together, our study strongly supported the fact that pterostilbene could be considered a safe and effective antifungal agent against A. flavus infection.<br />Competing Interests: Declaration of competing interest The authors affirm that they have no known competing financial interests or interpersonal conflicts that might have an impact on the research presented in this paper.<br /> (Copyright © 2023. Published by Elsevier B.V.)

Details

Language :
English
ISSN :
1879-3460
Volume :
404
Database :
MEDLINE
Journal :
International journal of food microbiology
Publication Type :
Academic Journal
Accession number :
37454507
Full Text :
https://doi.org/10.1016/j.ijfoodmicro.2023.110318