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The characteristic structure of funoran could be hydrolyzed by a GH86 family enzyme (Aga86A_Wa): Discovery of the funoran hydrolase.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2023 Oct 15; Vol. 318, pp. 121117. Date of Electronic Publication: 2023 Jun 10. - Publication Year :
- 2023
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Abstract
- Funoran, agarose and porphyran all belong to agaran, and share the similar skeleton. Although the glycoside hydrolase for agarose and porphyran, i.e. agarase and porphyranase, have been extensively studied, the enzyme hydrolyzing funoran has not been reported hitherto. The crystal structure of a previously characterized GH86 β-agarase Aga86A&#95;Wa showed a large cavity at subsite -1, which implied its ability to accommodate sulfate ester group. By using glycomics and NMR analysis, the activity of Aga86A&#95;Wa on the characteristic structure of funoran was validated, which signified the first discovery of funoran hydrolase, i.e. funoranase. Aga86A&#95;Wa hydrolyzed the β-1,4 glycosidic bond between β-d-galactopyranose-6-sulfate (G6S) and 3,6-anhydro-α-l-galactopyranose (LA) unit of funoran, and released disaccharide LA-G6S as the predominant end product. Considering the hydrolysis pattern, we proposed to name the activity represented by Aga86A&#95;Wa on funoran as "β-funoranase" and suggested to assign it an EC number.<br />Competing Interests: Declaration of competing interest The authors declare no conflict of interest.<br /> (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Subjects :
- Sepharose
Glycoside Hydrolases chemistry
Sulfates
Galactose
Polysaccharides
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 318
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 37479453
- Full Text :
- https://doi.org/10.1016/j.carbpol.2023.121117