Back to Search
Start Over
SNORA73B promotes endometrial cancer progression through targeting MIB1 and regulating host gene RCC1 alternative splicing.
- Source :
-
Journal of cellular and molecular medicine [J Cell Mol Med] 2023 Oct; Vol. 27 (19), pp. 2890-2905. Date of Electronic Publication: 2023 Jul 24. - Publication Year :
- 2023
-
Abstract
- Endometrial cancer (EC) is a common gynaecological malignant tumour with unclear pathogenesis. Small nucleolar RNA (snoRNA) is involved in many biological processes, including those of cancers. Using the Cancer Genome Atlas (TCGA) database, the expression pattern of a snoRNA, SNORA73B, was analysed. The biological functions of SNORA73B were assessed by in vitro proliferation, apoptosis, migration, and invasion assays and in vivo by the xenograft model. RNA sequencing (RNA-seq) and RNA immunoprecipitation assays were performed to determine the relationship between SNORA73B and its target genes. High-performance liquid chromatography (HPLC) was performed to detect the pseudouridine content of the mindbomb E3 ubiquitin protein ligase 1 gene (MIB1). The stability of MIB1 mRNA was evaluated using a transcription inhibitor, actinomycin D. By performing co-immunoprecipitation assays, the change in the ubiquitin levels of the Jagged canonical Notch ligand 1 (Jag 1), caused by SNORA73B and MIB1, was identified. RNA-seq and qRT-PCR were performed to detect the alternative splicing of the regulator of the chromosome condensation 1 gene (RCC1). The TCGA database analysis showed that SNORA73B was highly expressed in EC. SNORA73B promoted cell proliferation, migration, and invasion and inhibited apoptosis. SNORA73B modified the pseudouridine content in MIB1 and increased the stability of MIB1 mRNA and protein; thus, it affected Jag 1 ubiquitination and further activated the Notch pathway. SNORA73B also affected the alternative splicing of RCC1, increasing the number of transcripts, RCC1-T2 and RCC1-T3, which promoted cell proliferation, migration, and invasion. SNORA73B can be a potential target for EC.<br /> (© 2023 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.)
- Subjects :
- Female
Humans
Alternative Splicing genetics
Pseudouridine metabolism
RNA, Small Nucleolar genetics
RNA, Messenger metabolism
Cell Proliferation genetics
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Nuclear Proteins genetics
Cell Cycle Proteins metabolism
Guanine Nucleotide Exchange Factors genetics
Ubiquitin-Protein Ligases metabolism
Endometrial Neoplasms genetics
Endometrial Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1582-4934
- Volume :
- 27
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- Journal of cellular and molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 37488742
- Full Text :
- https://doi.org/10.1111/jcmm.17850