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YY1 Knockdown Relieves the Differentiation Block and Restores Apoptosis in AML Cells.

Authors :
Noguera NI
Travaglini S
Scalea S
Catalanotto C
Reale A
Zampieri M
Zaza A
Ricciardi MR
Angelini DF
Tafuri A
Ottone T
Voso MT
Zardo G
Source :
Cancers [Cancers (Basel)] 2023 Aug 07; Vol. 15 (15). Date of Electronic Publication: 2023 Aug 07.
Publication Year :
2023

Abstract

In this study we analyzed the expression of Yin and Yang 1 protein (YY1), a member of the noncanonical PcG complexes, in AML patient samples and AML cell lines and the effect of YY1 downregulation on the AML differentiation block. Our results show that YY1 is significantly overexpressed in AML patient samples and AML cell lines and that YY1 knockdown relieves the differentiation block. YY1 downregulation in two AML cell lines (HL-60 and OCI-AML3) and one AML patient sample restored the expression of members of the CEBP protein family, increased the expression of extrinsic growth factors/receptors and surface antigenic markers, induced morphological cell characteristics typical of myeloid differentiation, and sensitized cells to retinoic acid treatment and to apoptosis. Overall, our data show that YY1 is not a secondary regulator of myeloid differentiation but that, if overexpressed, it can play a predominant role in myeloid differentiation block.

Details

Language :
English
ISSN :
2072-6694
Volume :
15
Issue :
15
Database :
MEDLINE
Journal :
Cancers
Publication Type :
Academic Journal
Accession number :
37568827
Full Text :
https://doi.org/10.3390/cancers15154010