Discovery of Naphthol Tetramers from Endolichenic Fungus Daldinia childiae 047219 Based on MS/MS Molecular Networking.

Feature-based molecular networking analysis suggested the presence of naphthol tetramers in Daldinia childae 047219, the same species but a different strain from one used previously for the discovery of naphthol trimers promoting adiponectin synthesis. The new tetramers were composed of 5-methoxy-4-naphthol, each of which was connected to one another in various positions. Targeted isolation afforded six previously unreported naphthol tetramers ( 1 - 6 ) together with 13 known polyketides ( 7 - 19 ) including naphthol monomers, dimers, and trimers. Structures of the isolated compounds were established by using NMR and mass spectroscopic analysis. Nodulisporin A ( 13 ), nodulisporin B ( 14 ), and 1,1',3',3″-ternaphthalene-5,5',5″-trimethoxy-4,4',4″-triol ( 16 ) demonstrated anti-inflammatory activities against NO production, but the new compounds were less active.