Chemical Probe for Imaging of Polo-like Kinase 4 and Centrioles.

Authors :: Salim A
Werther P
Hatzopoulos GN
Reymond L
Wombacher R
Gönczy P
Johnsson K
Source :: JACS Au [JACS Au] 2023 Aug 04; Vol. 3 (8), pp. 2247-2256. Date of Electronic Publication: 2023 Aug 04 (Print Publication: 2023).
Publication Year :: 2023
Abstract: Polo-like kinase (Plk4) is a serine/threonine-protein kinase that is essential for biogenesis of the centriole organelle and is enriched at centrioles. Herein, we introduce Cen-TCO, a chemical probe based on the Plk4 inhibitor centrinone, to image Plk4 and centrioles in live or fixed cultured human cells. Specifically, we established a bio-orthogonal two-step labeling system that enables the Cen-TCO-mediated imaging of Plk4 by STED super-resolution microscopy. Such direct labeling of Plk4 results in an increased resolution in STED imaging compared with using anti-Plk4 antibodies, underlining the importance of direct labeling strategies for super-resolution microscopy. We anticipate that Cen-TCO will become an important tool for investigating the biology of Plk4 and of centrioles.<br />Competing Interests: The authors declare no competing financial interest.<br /> (© 2023 The Authors. Published by American Chemical Society.)