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Utilization of an immunochromatographic lateral flow assay for rapid detection of carbapenemase production in gram negative bacilli.
- Source :
-
Laboratory medicine [Lab Med] 2023 Nov 02; Vol. 54 (6), pp. e204-e206. - Publication Year :
- 2023
-
Abstract
- Background: Rapid detection of carbapenemase production in gram negative bacilli has important treatment considerations.<br />Objective: We evaluated a lateral flow assay (LFA) for carbapenemase production compared with molecular detection of 5 (blaKPC, blaNDM, blaVIM, blaIMP, and blaOXA-48) carbapenemase genes.<br />Methods: A total of 218 carbapenem nonsusceptible strains, including species of Enterobacterales, Pseudomonas aeruginosa isolated from clinical cultures were tested using the Cepheid Xpert Carba-R assay and the NG Biotech Carba-5 lateral flow immunoassay.<br />Results: Overall agreement with LFA was 98.2% with accuracy for each target >99% compared with polymerase chain reaction. Results were available within 15 minutes compared with 1 hour for molecular detection.<br />Conclusion: The use of accurate, rapid diagnostics compliments antimicrobial stewardship programs.<br /> (© The Author(s) 2023. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Details
- Language :
- English
- ISSN :
- 1943-7730
- Volume :
- 54
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Laboratory medicine
- Publication Type :
- Academic Journal
- Accession number :
- 37658816
- Full Text :
- https://doi.org/10.1093/labmed/lmad090