Role of some inflammasomes in rheumatoid arthritis patients in Egypt.

Authors :: Ibrahem R
Raghip MA
Abdelwahed MM
Amin NS
Abualfadl EM
Waly NGFM
Source :: Molecular biology reports [Mol Biol Rep] 2023 Nov; Vol. 50 (11), pp. 8809-8815. Date of Electronic Publication: 2023 Sep 02.
Publication Year :: 2023
Abstract: Aim: This study aims to demonstrate the role of some inflammasomes genes: NLRC4 (the NLR family, CARD domain-containing protein 4), NLRP1 (NLR family, pyrin domain-containing 1), ASC (Apoptosis-associated speck-like protein containing a CARD), and CASPASE-1 in the pathogenesis of Rheumatoid arthritis (RA) in Egyptian population.<br />Main Methods: The expression level of NLRC4, NLRP1, ASC, and CASPASE-1 within PBMCs isolated from all RA subjects by quantitative real-time PCR. GAPDH gene was used as a reference gene. Measurement of serum level of IL-1β and IL-18 was performed using ELISA.<br />Key Findings: Results showed dysregulated inflammasomes expression that may participate in the pathogenesis of the inflammatory process of the disease.<br />Significance: Understanding the role of inflammasomes in RA pathogenesis helps in finding promising therapy for the treatment and management of this disease.<br /> (© 2023. The Author(s).)