A protocol for whole-brain Ca 2+ imaging in Astyanax mexicanus, a model of comparative evolution.

Authors :: Lloyd E
Privat M
Sumbre G
Duboué ER
Keene AC
Source :: STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102517. Date of Electronic Publication: 2023 Sep 23.
Publication Year :: 2023
Abstract: In this protocol, we describe a comparative approach to study the evolution of brain function in the Mexican tetra, Astyanax mexicanus. We developed surface fish and two independent populations of cavefish with pan-neuronal expression of the Ca <superscript>2+</superscript> sensor GCaMP6s. We describe a methodology to prepare samples and image activity across the optic tectum and olfactory bulb.<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

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