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Neuraminidase-dependent entry of influenza A virus is determined by hemagglutinin receptor-binding specificity.

Authors :
Wallace LE
de Vries E
van Kuppeveld FJM
de Haan CAM
Source :
Journal of virology [J Virol] 2023 Oct 31; Vol. 97 (10), pp. e0060223. Date of Electronic Publication: 2023 Sep 27.
Publication Year :
2023

Abstract

Importance: Influenza A viruses (IAVs) contain hemagglutinin (HA) proteins involved in sialoglycan receptor binding and neuraminidase (NA) proteins that cleave sialic acids. While the importance of the NA protein in virion egress is well established, its role in virus entry remains to be fully elucidated. NA activity is needed for the release of virions from mucus decoy receptors, but conflicting results have been reported on the importance of NA activity in virus entry in the absence of decoy receptors. We now show that inhibition of NA activity affects virus entry depending on the receptor-binding properties of HA and the receptor repertoire present on cells. Inhibition of entry by the presence of mucus correlated with the importance of NA activity for virus entry, with the strongest inhibition being observed when mucus and OsC were combined. These results shed light on the importance in virus entry of the NA protein, an important antiviral drug target.<br />Competing Interests: The authors declare no conflict of interest.

Details

Language :
English
ISSN :
1098-5514
Volume :
97
Issue :
10
Database :
MEDLINE
Journal :
Journal of virology
Publication Type :
Academic Journal
Accession number :
37754760
Full Text :
https://doi.org/10.1128/jvi.00602-23