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Human IgM hi CD300a + B Cells Are Circulating Marginal Zone Memory B Cells That Respond to Pneumococcal Polysaccharides and Their Frequency Is Decreased in People Living with HIV.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2023 Sep 06; Vol. 24 (18). Date of Electronic Publication: 2023 Sep 06. - Publication Year :
- 2023
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Abstract
- CD300a is differentially expressed among B cell subsets, although its expression in immunoglobulin (Ig)M <superscript>+</superscript> B cells is not well known. We identified a B cell subset expressing CD300a and high levels of IgM (IgM <superscript>hi</superscript> CD300a <superscript>+</superscript> ). The results showed that IgM <superscript>hi</superscript> CD300a <superscript>+</superscript> B cells were CD10 <superscript>-</superscript> CD27 <superscript>+</superscript> CD25 <superscript>+</superscript> IgD <superscript>lo</superscript> CD21 <superscript>hi</superscript> CD23 <superscript>-</superscript> CD38 <superscript>lo</superscript> CD1c <superscript>hi</superscript> , suggesting that they are circulating marginal zone (MZ) IgM memory B cells. Regarding the immunoglobulin repertoire, IgM <superscript>hi</superscript> CD300a <superscript>+</superscript> B cells exhibited a higher mutation rate and usage of the IgH-VDJ genes than the IgM <superscript>+</superscript> CD300a <superscript>-</superscript> counterpart. Moreover, the shorter complementarity-determining region 3 (CDR3) amino acid (AA) length from IgM <superscript>hi</superscript> CD300a <superscript>+</superscript> B cells together with the predicted antigen experience repertoire indicates that this B cell subset has a memory phenotype. IgM memory B cells are important in T cell-independent responses. Accordingly, we demonstrate that this particular subset secretes higher amounts of IgM after stimulation with pneumococcal polysaccharides or a toll-like receptor 9 (TLR9) agonist than IgM <superscript>+</superscript> CD300a <superscript>-</superscript> cells. Finally, the frequency of IgM <superscript>hi</superscript> CD300a <superscript>+</superscript> B cells was lower in people living with HIV-1 (PLWH) and it was inversely correlated with the years with HIV infection. Altogether, these data help to identify a memory B cell subset that contributes to T cell-independent responses to pneumococcal infections and may explain the increase in severe pneumococcal infections and the impaired responses to pneumococcal vaccination in PLWH.
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 24
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 37762055
- Full Text :
- https://doi.org/10.3390/ijms241813754