Occurrence, stability and cytotoxicity of halobenzamides: A new group of nitrogenous disinfection byproducts in drinking water.

Exploring disinfection byproducts (DBPs) with adverse health effects in drinking water is a constant challenge. Halobenzamides (HBZAMs) are suspected to be a new group of nitrogenous DBPs but have not been reported in drinking water to date. In this study, by coupling SPE and UPLC‒MS/MS, a sensitive method was established to detect eight HBZAMs in drinking water with recoveries and limits of detection of 80-103% and 0.01-0.04 ng/L, respectively. Subsequently, distinct fragments of HBZAMs were extended to the development of a pseudotargeted method for the analysis of the fourteen HBZAMs that were speculated and lack chemical standards. Using the developed method, eight HBZAMs were quantified in ten drinking water samples with concentrations ranging from 2.4 to 7.2 ng/L and a detection frequency of 100%, among which five HBZAMs were stable with half-lives over 72 h under real chlorine levels. Twelve HBZAMs without standards were identified in three to ten drinking water samples with comparable levels. The cytotoxicity of eight quantified HBZAMs in CHO-K1 cells varied with disparity, in which the cytotoxicity of 3,5-DBBZAM was over 10-fold higher than that of aliphatic dichloroacetamide. Considering their diversity, toxicity and stability, the occurrence of HBZAMs in drinking water deserves attention.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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