Back to Search
Start Over
Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source.
- Source :
-
IUCrJ [IUCrJ] 2023 Nov 01; Vol. 10 (Pt 6), pp. 729-737. Date of Electronic Publication: 2023 Nov 01. - Publication Year :
- 2023
-
Abstract
- Serial and time-resolved macromolecular crystallography are on the rise. However, beam time at X-ray free-electron lasers is limited and most third-generation synchrotron-based macromolecular crystallography beamlines do not offer the necessary infrastructure yet. Here, a new setup is demonstrated, based on the JUNGFRAU detector and Jungfraujoch data-acquisition system, that enables collection of kilohertz serial crystallography data at fourth-generation synchrotrons. More importantly, it is shown that this setup is capable of collecting multiple-time-point time-resolved protein dynamics at kilohertz rates, allowing the probing of microsecond to second dynamics at synchrotrons in a fraction of the time needed previously. A high-quality complete X-ray dataset was obtained within 1 min from lysozyme microcrystals, and the dynamics of the light-driven sodium-pump membrane protein KR2 with a time resolution of 1 ms could be demonstrated. To make the setup more accessible for researchers, downstream data handling and analysis will be automated to allow on-the-fly spot finding and indexing, as well as data processing.<br /> (open access.)
Details
- Language :
- English
- ISSN :
- 2052-2525
- Volume :
- 10
- Issue :
- Pt 6
- Database :
- MEDLINE
- Journal :
- IUCrJ
- Publication Type :
- Academic Journal
- Accession number :
- 37830774
- Full Text :
- https://doi.org/10.1107/S2052252523008618