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Transcriptional regulation and overexpression of GST cluster enhances pesticide resistance in the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae).
- Source :
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Communications biology [Commun Biol] 2023 Oct 19; Vol. 6 (1), pp. 1064. Date of Electronic Publication: 2023 Oct 19. - Publication Year :
- 2023
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Abstract
- The rapid evolution of resistance in agricultural pest poses a serious threat to global food security. However, the mechanisms of resistance through metabolic regulation are largely unknown. Here, we found that a GST gene cluster was strongly selected in North China (NTC) population, and it was significantly genetically-linked to lambda-cyhalothrin resistance. Knockout of the GST cluster using CRISPR/Cas9 significantly increased the sensitivity of the knockout strain to lambda-cyhalothrin. Haplotype analysis revealed no non-synonymous mutations or structural variations in the GST cluster, whereas GST&#95;119 and GST&#95;121 were significantly overexpressed in the NTC population. Silencing of GST&#95;119 or co-silencing of GST&#95;119 and GST&#95;121 with RNAi significantly increased larval sensitivity to lambda-cyhalothrin. We also identified additional GATAe transcription factor binding sites in the promoter of NTC&#95;GST&#95;119. Transient expression of GATAe in Hi5 cells activated NTC&#95;GST&#95;119 and Xinjiang (XJ)&#95;GST&#95;119 transcription, but the transcriptional activity of NTC&#95;GST&#95;119 was significantly higher than that of XJ&#95;GST&#95;119. These results demonstrate that variations in the regulatory region result in complex expression changes in the GST cluster, which enhances lambda-cyhalothrin resistance in field-populations. This study deepens our knowledge of the evolutionary mechanism of pest adaptation under environmental stress and provides potential targets for monitoring pest resistance and integrated management.<br /> (© 2023. Springer Nature Limited.)
Details
- Language :
- English
- ISSN :
- 2399-3642
- Volume :
- 6
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Communications biology
- Publication Type :
- Academic Journal
- Accession number :
- 37857697
- Full Text :
- https://doi.org/10.1038/s42003-023-05447-0