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Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes.
- Source :
-
Acta crystallographica. Section D, Structural biology [Acta Crystallogr D Struct Biol] 2023 Nov 01; Vol. 79 (Pt 11), pp. 1018-1025. Date of Electronic Publication: 2023 Nov 01. - Publication Year :
- 2023
-
Abstract
- In recent years, the emergence of serial crystallography, initially pioneered at X-ray free-electron lasers (XFELs), has sparked a growing interest in collecting macromolecular crystallographic data at room temperature. Various fixed-target serial crystallography techniques have been developed, ranging from commercially available chips to in-house designs implemented at different synchrotron facilities. Nevertheless, there is currently no commercially available chip (known to the authors) specifically designed for the direct handling of oxygen-sensitive samples. This study presents a methodology employing silicon nitride chips arranged in a `sandwich' configuration, enabling reliable room-temperature data collection from oxygen-sensitive samples. The method involves the utilization of a custom-made 3D-printed assembling tool and a MX sample holder. To validate the effectiveness of the proposed method, deoxyhemoglobin and methemoglobin samples were investigated using the BioMAX X-ray macromolecular crystallography beamline, the Balder X-ray absorption spectroscopy beamline and UV-Vis absorption spectroscopy.<br /> (open access.)
Details
- Language :
- English
- ISSN :
- 2059-7983
- Volume :
- 79
- Issue :
- Pt 11
- Database :
- MEDLINE
- Journal :
- Acta crystallographica. Section D, Structural biology
- Publication Type :
- Academic Journal
- Accession number :
- 37860963
- Full Text :
- https://doi.org/10.1107/S205979832300880X