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Histatin-5 interacts with cellular copper to promote antifungal activity against Candida albicans.
- Source :
-
Metallomics : integrated biometal science [Metallomics] 2023 Dec 09; Vol. 15 (12). - Publication Year :
- 2023
-
Abstract
- Histatin-5 (Hist-5) is an antimicrobial peptide found in human saliva that functions to defend the oral cavity from microbial infections, such as those caused by the fungal pathogen Candida albicans (C. albicans). Hist-5 can bind Cu in multiple oxidation states, Cu2+ and Cu+in vitro, and supplemental Cu2+ has been shown to improve the fungicidal activity of the peptide against C. albicans in culture. However, the exact role of Cu on the antifungal activity of Hist-5 and whether direct peptide-Cu interactions occur intracellularly has yet to be fully determined. Here, we used a combination of fluorescence spectroscopy and confocal microscopy experiments to show reversible Cu-dependent quenching of a fluorescent Hist-5 analogue, Hist-5*, indicating a direct interaction between Hist-5 and intracellular Cu. X-ray fluorescence microscopy images revealed peptide-induced changes to cellular Cu distribution and cell-associated Cu content. These data support a model in which Hist-5 can facilitate the hyperaccumulation of Cu in C. albicans and directly interact with Cu intracellularly to increase the fungicidal activity of Hist-5.<br /> (© The Author(s) 2023. Published by Oxford University Press.)
Details
- Language :
- English
- ISSN :
- 1756-591X
- Volume :
- 15
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Metallomics : integrated biometal science
- Publication Type :
- Academic Journal
- Accession number :
- 38061812
- Full Text :
- https://doi.org/10.1093/mtomcs/mfad070