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Endothelial Progenitor-Cell-Derived Exosomes Induced by Astragaloside IV Accelerate Type I Diabetic-wound Healing via the PI3K/AKT/mTOR Pathway in Rats.
- Source :
-
Frontiers in bioscience (Landmark edition) [Front Biosci (Landmark Ed)] 2023 Nov 08; Vol. 28 (11), pp. 282. - Publication Year :
- 2023
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Abstract
- Objective: We explore the effects of endothelial progenitor cell (EPC)-derived exosomes (EPCexos) and of astragaloside IV (ASIV)-stimulated EPCexos (ASIV-EPCexos) on type I diabetic-wound healing, and determine the basic molecular mechanisms of action.<br />Methods: EPCs were exposed to different concentrations of ASIV to generate ASIV-EPCexos. A chronic-wound healing model involving streptozotocin-stimulated diabetic rats was established. These rats were treated with EPCexos, ASIV-EPCexos, rapamycin, and wortmannin. Wound healing was evaluated by direct photographic observation, hematoxylin and eosin staining, and Masson's trichrome staining.<br />Results: ASIV treatment increased the abilities of EPCs (e.g., proliferation), as well as exosome secretion. EPCexo showed a "cup holder" like structure. Treatment with ASIV-EPCexos increased the wound-healing rate, collagen-deposition area, bromodeoxyuridine uptake, VEGF expression, and the number of CD31- and αSMA- positive cells, whereas decreased epidermal thickness and CD45 expression. The expression of the PI3K/AKT/mTOR pathway increased, whereas the expression of inflammatory factor decreased. However, rapamycin and wortmannin reversed these changes.<br />Conclusions: ASIV-EPCexos may accelerate type I diabetic-wound healing via the PI3K/AKT/mTOR pathway. This study may lay the foundation for new clinical treatment options for patients with type I diabetic wounds.<br />Competing Interests: The authors declare no conflict of interest.<br /> (© 2023 The Author(s). Published by IMR Press.)
- Subjects :
- Animals
Rats
Phosphatidylinositol 3-Kinases metabolism
Proto-Oncogene Proteins c-akt metabolism
Rats, Sprague-Dawley
Sirolimus pharmacology
TOR Serine-Threonine Kinases metabolism
Wortmannin metabolism
Wortmannin pharmacology
Wound Healing
Diabetes Mellitus, Experimental drug therapy
Diabetes Mellitus, Experimental metabolism
Diabetes Mellitus, Type 1 metabolism
Endothelial Progenitor Cells metabolism
Exosomes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2768-6698
- Volume :
- 28
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Frontiers in bioscience (Landmark edition)
- Publication Type :
- Academic Journal
- Accession number :
- 38062822
- Full Text :
- https://doi.org/10.31083/j.fbl2811282