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Saxagliptin reduces the injury of Alzheimer's disease cell model by down-regulating the expression of miR-483-5p.
- Source :
-
Cellular and molecular biology (Noisy-le-Grand, France) [Cell Mol Biol (Noisy-le-grand)] 2023 Nov 30; Vol. 69 (12), pp. 188-193. Date of Electronic Publication: 2023 Nov 30. - Publication Year :
- 2023
-
Abstract
- This study aimed to investigate the effect of saxagliptin on the injury of Alzheimer's disease (AD) cell model and its possible mechanism. SK-N-SH cells were cultured in vitro and divided into CON group, AD group, AD+L-SAX group, AD+M-SAX group, AD+H-SAX group, AD+anti-miR-NC group, AD+anti-miR-483-5p group, AD+SAX+miR-NC group and AD+SAX+miR-483-5p group. Then the levels of MDA, SOD and GSH-Px in each group were detected by ELISA method; cell apoptosis was detected by flow cytometry; the protein expression levels of Bax and Bcl-2 were detected by Western Blot; the expression level of miR-483-5p was detected by RT-qPCR. Compared with the control group, MDA content, apoptosis rate, and the expression of Bax protein and miR-483-5p increased in the AD group (P<0.05), while the activity of SOD and GSH-Px and the expression of Bcl-2 protein decreased (P<0.05). Compared with the AD group, MDA content, apoptosis rate, and the expression of Bax protein and miR-483-5p decreased in the AD+L-SAX group, AD+M-SAX group and AD+H-SAX group (P<0.05), while the activity of SOD and GSH-Px and the expression of Bcl-2 protein increased (P<0.05). Compared with AD+anti-miR-NC group, MDA content, apoptosis rate, and the expression of Bax protein and miR-483-5p decreased in the AD+anti-miR-483-5p group (P<0.05), while the activity of SOD and GSH-Px and the expression of Bcl-2 protein increased (P<0.05). Compared with AD+SAX+miR-NC group, MDA content, apoptosis rate, and the expression of Bax protein and miR-483-5p increased in the AD+SAX+miR-483-5p group (P<0.05), while the activity of SOD and GSH-Px and the expression of Bcl-2 protein decreased (P<0.05). Saxagliptin may reduce the injury of Alzheimer's disease cell model by down-regulating the expression of miR-483-5p.
- Subjects :
- Humans
bcl-2-Associated X Protein genetics
bcl-2-Associated X Protein metabolism
Antagomirs
Proto-Oncogene Proteins c-bcl-2 genetics
Proto-Oncogene Proteins c-bcl-2 metabolism
Apoptosis genetics
Superoxide Dismutase genetics
Alzheimer Disease drug therapy
Alzheimer Disease genetics
MicroRNAs metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1165-158X
- Volume :
- 69
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Cellular and molecular biology (Noisy-le-Grand, France)
- Publication Type :
- Academic Journal
- Accession number :
- 38063096
- Full Text :
- https://doi.org/10.14715/cmb/2023.69.12.30