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The cardioprotective effects of cerium oxide nanoparticles against the poisoning generated by aluminum phosphide pesticide: Controlling oxidative stress and mitochondrial damage.
- Source :
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Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2023 Dec; Vol. 197, pp. 105701. Date of Electronic Publication: 2023 Nov 20. - Publication Year :
- 2023
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Abstract
- Background: Aluminum phosphide (AlP) is a well-known toxic compound used as an agricultural pesticide to prevent insect damage to stored crops. However, even if just a small amount was consumed, it caused lasting harm to the human body and, in acute concentrations, death. The current study employed cerium oxide nanoparticles (CeO <subscript>2</subscript> NPs) to reduce oxidative stress and various harmful outcomes of AlP poisoning.<br />Methods: Following finding effective concentrations of CeO <subscript>2</subscript> NPs via MTT assay, Human Cardiac Myocyte (HCM) cells were pre-treated with CeO <subscript>2</subscript> NPs for 24 h. After that, they were exposed to 2.36 μM AlP. The activity of oxidative stress and mitochondrial biomarkers, including mitochondrial swelling, mitochondrial membrane potential, and cytochrome c release, were evaluated in HCM cells. Finally, the population of apoptotic and necrotic cells was assessed via flow cytometry.<br />Results: After 24 h, data revealed that all tested concentrations of CeO <subscript>2</subscript> NPs were safe, and 25 and 50 μM of that were selected as effective concentrations. Oxidative stress markers (malondialdehyde, protein carbonyl, superoxide dismutase, and catalase) showed that CeO <subscript>2</subscript> NPs could successfully decrease AlP poisoning due to their antioxidant characteristics. Mitochondrial markers were also recovered by pre-treatment of HCM cells with CeO <subscript>2</subscript> NPs. Furthermore, pre-treating with CeO <subscript>2</subscript> NPs could compensate for the reduction of live cells with AlP and cause a diminishing in the population of early and late apoptotic cells.<br />Conclusion: As a result, it is evident that CeO <subscript>2</subscript> NPs, through the recovery of oxidative stress and mitochondrial damages caused by AlP, reduce apoptosis and have therapeutic potentials on HCM cells.<br />Competing Interests: Declaration of Competing Interest The authors declare that there are no conflicts of interest.<br /> (Copyright © 2023. Published by Elsevier Inc.)
- Subjects :
- Humans
Oxidative Stress
Pesticides toxicity
Nanoparticles
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9939
- Volume :
- 197
- Database :
- MEDLINE
- Journal :
- Pesticide biochemistry and physiology
- Publication Type :
- Academic Journal
- Accession number :
- 38072556
- Full Text :
- https://doi.org/10.1016/j.pestbp.2023.105701