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Up-regulation of miR-155 protects against chronic heart failure by inhibiting HIF-1α.
- Source :
-
American journal of translational research [Am J Transl Res] 2023 Nov 15; Vol. 15 (11), pp. 6425-6436. Date of Electronic Publication: 2023 Nov 15 (Print Publication: 2023). - Publication Year :
- 2023
-
Abstract
- Background: Despite a crucial role of miR-155 in human cancers, its function in heart failure (HF) is still under investigation. This study was designed to explore its association with HF.<br />Methods: The abdominal transverse aortic constriction (TAC) was adopted for establishment of mouse HF models. qRT-PCR and WB were adopted to detect the changes of miR-155, HIF-1α, Cle-caspase-3, BCL2 and Bax levels in myocardial cells and heart tissues. The changes of cardiac function were checked by ultrasound. Additionally, luciferase reporter gene was adopted for interaction analysis of miR-155 with HIF-1α, and in situ end labelling method was used for detecting myocardial apoptosis.<br />Results: MiR-155 in myocardial tissue of HF mice was significantly down regulated. In HF mice injected with agomiR-155, the up-regulation of miR-155 strongly improved cardiac function, and also significantly lowered the protein levels of apoptosis-associated markers, C-caspase-3 and Bax, but up regulated Bcl-2. Additionally, HIF-1α was identified as the direct target of miR-155. As expected, over-expression of HIF-1α greatly reversed the effects of agomiR-155 on cardiac function and the expression of apoptosis-associated markers in heart tissues of HF mice.<br />Conclusion: MiR-155 overexpression can suppress myocardial cell apoptosis through HIF-1α, and strongly alleviate the cardiac function damage in HF mice, indicating the potential of miR-155/HIF-1α axis to be a target for the diagnosis and therapy of HF.<br />Competing Interests: None.<br /> (AJTR Copyright © 2023.)
Details
- Language :
- English
- ISSN :
- 1943-8141
- Volume :
- 15
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- American journal of translational research
- Publication Type :
- Academic Journal
- Accession number :
- 38074801