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LINC00115 promotes gastric cancer partly by the miR-212-5p/ATPAF1 axis.
- Source :
-
Anais da Academia Brasileira de Ciencias [An Acad Bras Cienc] 2023 Dec 11; Vol. 95 (suppl 2), pp. e20230480. Date of Electronic Publication: 2023 Dec 11 (Print Publication: 2023). - Publication Year :
- 2023
-
Abstract
- LncRNAs are known to be key regulators in the initiation and development of diverse cancers. Whether LINC00115 is involved in the regulation of gastric cancer (GC) progression remains unclear. Here, we aimed to show the function of LINC00115 in GC. RT-qPCR was used to measure gene expression in GC tissues and cells. Colony formation, EdU, TUNEL, and wound healing assays were used to analyze cellular processes in GC. The in vivo GC xenograft model was established. We observed that LINC00115 was highly expressed in GC. Functionally, silencing LINC00115 inhibited GC cell proliferation, and migration but facilitated GC apoptosis. Mechanistically, LINC00115 sponged miR-212-5p, while miR-212-5p targeted ATPAF1 in GC cells. Rescue assays showed ATPAF1 overexpression countervailed the inhibitory role of LINC00115 depletion in GC progression in vitro and in vivo. Overall, LINC00115 promoted GC progression by upregulating ATPAF1 via miR-212-5p.
Details
- Language :
- English
- ISSN :
- 1678-2690
- Volume :
- 95
- Issue :
- suppl 2
- Database :
- MEDLINE
- Journal :
- Anais da Academia Brasileira de Ciencias
- Publication Type :
- Academic Journal
- Accession number :
- 38088732
- Full Text :
- https://doi.org/10.1590/0001-3765202320230480