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Neuroprotective effect of whey protein hydrolysate containing leucine-aspartate-isoleucine-glutamine-lysine on HT22 cells in hydrogen peroxide-induced oxidative stress.
- Source :
-
Journal of dairy science [J Dairy Sci] 2024 May; Vol. 107 (5), pp. 2620-2632. Date of Electronic Publication: 2023 Dec 14. - Publication Year :
- 2024
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Abstract
- This study aimed to investigate the neuroprotective effects of whey protein hydrolysate (WPH) containing the pentapeptide leucine-aspartate-isoleucine-glutamine-lysine (LDIQK). Whey protein hydrolysate (50, 100, and 200 µg/mL) demonstrated the ability to restore the viability of HT22 cells subjected to 300 µM hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> )-induced oxidative stress. Furthermore, at a concentration of 200 µg/mL, it significantly reduced the increase in reactive oxygen species production and calcium ion (Ca <superscript>2+</superscript> ) influx induced by H <subscript>2</subscript> O <subscript>2</subscript> by 46.1% and 46.2%, respectively. Similarly, the hydrolysate significantly decreased the levels of p-tau, a hallmark of tauopathy, and BCL2 associated X (BAX), a proapoptosis factor, while increasing the protein levels of choline acetyltransferase (ChAT), an enzyme involved in acetylcholine synthesis, brain-derived neurotrophic factor (BDNF), a nerve growth factor, and B-cell lymphoma 2 (BCL2, an antiapoptotic factor. Furthermore, it increased nuclear factor erythroid 2-related factor 2 (Nrf2)-hemoxygenase-1(HO-1) signaling, which is associated with the antioxidant response, while reducing the activation of mitogen-activated protein kinase (MAPK) signaling pathway components, namely phosphor-extracellular signal-regulated kinases (p-ERK), phosphor-c-Jun N-terminal kinases (p-JNK), and p-p38. Column chromatography and tandem mass spectrometry analysis identified LDIQK as a compound with neuroprotective effects in WPH; it inhibited Ca <superscript>2+</superscript> influx and regulated the BAX/BCL2 ratio. Collectively, WPH containing LDIQK demonstrated neuroprotective effects against H <subscript>2</subscript> O <subscript>2</subscript> -induced neuronal cell damage, suggesting that WPH or its active peptide, LDIQK, may serve as a potential edible agent for improving cognitive dysfunction.<br /> (The Authors. Published by Elsevier Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).)
- Subjects :
- Animals
Glutamine pharmacology
Aspartic Acid metabolism
Aspartic Acid pharmacology
Isoleucine metabolism
Leucine metabolism
Lysine metabolism
Protein Hydrolysates pharmacology
Protein Hydrolysates metabolism
bcl-2-Associated X Protein metabolism
bcl-2-Associated X Protein pharmacology
Whey metabolism
Oxidative Stress
Reactive Oxygen Species metabolism
Extracellular Signal-Regulated MAP Kinases metabolism
Hydrogen Peroxide pharmacology
Neuroprotective Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1525-3198
- Volume :
- 107
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of dairy science
- Publication Type :
- Academic Journal
- Accession number :
- 38101744
- Full Text :
- https://doi.org/10.3168/jds.2023-24284