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The effect of aquaporin-4 inhibition on cerebrospinal fluid-tissue water exchange in mouse brain detected by magnetization transfer indirect spin labeling MRI.
- Source :
-
NMR in biomedicine [NMR Biomed] 2024 Jul; Vol. 37 (7), pp. e5093. Date of Electronic Publication: 2024 Jan 01. - Publication Year :
- 2024
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Abstract
- The fluid transport of cerebrospinal fluid (CSF) and interstitial fluid in surrounding tissues plays an important role in the drainage pathway that facilitates waste clearance from the brain. This pathway is known as the glymphatic or perivascular system, and its functions are dependent on aquaporin-4 (AQP4). Recently, magnetization transfer indirect spin labeling (MISL) magnetic resonance imaging (MRI) has been proposed as a noninvasive and noncontrast-enhanced method for detecting water exchange between CSF and brain tissue. In this study, we first optimized the MISL sequence at preclinical 3 T MRI, and then studied the correlation of MISL in CSF with magnetization transfer (MT) in brain tissue, as well as the altered water exchange under AQP4 inhibition, using C57BL/6 mice. Results showed a strong correlation of MISL signal with MT signal. With the AQP4 inhibitor, we observed a significant decrease in MISL value (Pā<ā0.05), suggesting that the hampered AQP4 activity led to decreased water exchange between CSF and brain tissue or the impairment of the glymphatic function. Overall, our findings demonstrate the potential application of MISL in assessing brain water exchange at 3 T MRI and its potential clinical translation.<br /> (© 2024 John Wiley & Sons Ltd.)
- Subjects :
- Animals
Mice
Water metabolism
Male
Body Water metabolism
Niacinamide analogs & derivatives
Thiadiazoles
Aquaporin 4 metabolism
Aquaporin 4 antagonists & inhibitors
Magnetic Resonance Imaging
Spin Labels
Mice, Inbred C57BL
Brain diagnostic imaging
Brain metabolism
Cerebrospinal Fluid metabolism
Cerebrospinal Fluid diagnostic imaging
Subjects
Details
- Language :
- English
- ISSN :
- 1099-1492
- Volume :
- 37
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- NMR in biomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 38163739
- Full Text :
- https://doi.org/10.1002/nbm.5093