In vitro study on the transmission of multidrug-resistant bacteria from textiles to pig skin.

Background: The survival of microorganisms on textiles and specifically on healthcare professionals' (HCP) attire has been demonstrated in several studies. The ability of microorganisms to adhere and remain on textiles for up to hours or days raises questions as to their possible role in transmission from textile to skin via HCP to patients.
Aim: To evaluate the presence, survival and transmission of different multidrug-resistant bacteria (MDRB) from HCP attire onto skin.
Methods: Three MDRB [methicillin-resistant Staphylococcus aureus (MRSA); vancomycin-resistant Enterococcus faecium (VRE) ; carbapenem-resistant Klebsiella pneumoniae , CRKP)] were inoculated on textiles from scrubs (60% cotton-40% polyester) and white coat (100% cotton) at concentrations of 10 ⁸ colony-forming units (CFU), 10 ⁵ CFU, and 10 ³ CFU per mL. The inoculation of swatches was divided in time intervals of 1 min, 5 min, 15 min, 30 min, 1 h, 2 h, 3 h, 4 h, 5 h, and 6 h. At the end of each period, textiles were imprinted onto pig skins and each skin square was inverted onto three different selective chromogenic media. Growth from the pig skin squares was recorded for the 3 MDRB at the three above concentrations, for the whole length of the 6-h experiment.
Results: MRSA was recovered from pig skins at all concentrations for the whole duration of the 6-h study. VRE was recovered from the concentration of 10 ⁸ CFU/mL for 6 h and from 10 ⁵ CFU/mL for up to 3 h, while showing no growth at 10 ³ CFU/mL . CRKP was recovered from 10 ⁸ CFU/mL for 6 h, up to 30 min from 10 ⁵ CFU/mL and for 1 min from the concentration of 10 ³ CFU/mL.
Conclusion: Evidence from the current study shows that MRSA can persist on textiles and transmit to skin for 6 h even at low concentrations. The fact that all MDRB can be sustained and transferred to skin even at lower concentrations, supports that textiles are implicated as vectors of bacterial spread.
Competing Interests: Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
(©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.)