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Cryoprotective Effect of Pectin Tanacetan from Tanacetum vulgare L.
- Source :
-
Biopreservation and biobanking [Biopreserv Biobank] 2024 Aug; Vol. 22 (4), pp. 336-345. Date of Electronic Publication: 2024 Jan 08. - Publication Year :
- 2024
-
Abstract
- We researched the ability of tanacetan pectin from inflorescences of common tansy Tanacetum vulgare L. to change the osmolarity and freezing point of water in solutions of cryoprotectants: glycerol-3.5%, dimethyl sulfoxide (DMSO)-10%, dimethylacetamide-10% (DMAC), and 1.2-propanediol (1.2-PD)-10%, as well as the effect of solutions of tanacetan (0.2%, 0.4%) on the kinetics of crystallization processes and the nature of crystal formation. We used a combination of protector and pectin that we tested earlier, which provided effective protection for human leukocytes and platelets, as well as bovine spermatozoa, at temperatures below freezing (-20°C and -80°C). It has been established that tanacetan slows down the process of water freezing in glycerol, but not in DMSO, DMAC, and 1.2-PD, promotes deeper supercooling of the medium, and affects the morphological structure of ice. The addition of pectin to the cryosolution increases the activity of the main cryoprotectant glycerol even at its low concentrations. The combination of glycerol and tanacetan can be effective in freezing biological materials, which is confirmed by the preservation of leukocytes at -20°C and -80°C for 7 days, platelets at -80°C for 30 days, and spermatozoa at -80°C within 1 day. A comprehensive analysis of the chemical, physicochemical, and cryoprotective properties of tanacetan indicates the prospect of using pectin in the cryopreservation of biological objects at temperatures of electric freezers.
- Subjects :
- Humans
Male
Animals
Cattle
Freezing
Dimethyl Sulfoxide pharmacology
Dimethyl Sulfoxide chemistry
Leukocytes drug effects
Leukocytes cytology
Spermatozoa drug effects
Blood Platelets drug effects
Cryoprotective Agents pharmacology
Cryoprotective Agents chemistry
Pectins pharmacology
Pectins chemistry
Cryopreservation methods
Glycerol pharmacology
Glycerol chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1947-5543
- Volume :
- 22
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biopreservation and biobanking
- Publication Type :
- Academic Journal
- Accession number :
- 38190112
- Full Text :
- https://doi.org/10.1089/bio.2023.0066