Ziziphora clinopodioides extract-loaded chitosan/polyvinylpyrrolidone casting films for potential applications in wound dressings.

Chitosan/polyvinylpyrrolidone composite films containing hydroalcoholic Ziziphora clinopodioides extract were developed and evaluated for their potential use as wound dressings. The physical and chemical properties of the films were extensively explored, including swelling capacity, mechanical properties, antimicrobial activity, and microstructural characteristics. The results showed that the addition of Ziziphora extract significantly increased the swelling capacity of the films by 561.24% to 1175% ( p  < 0.05). While tensile strength and Young's modulus were enhanced, elongation at the breaking point decreased with increasing volume percentages of Ziziphora extract. Variance analysis indicated no statistically significant effect on the tensile properties of the films with varying Ziziphora extract content ( p  < 0.05). Furthermore, films incorporated with Ziziphora extract demonstrated antimicrobial properties. Scanning electron microscopy (SEM) analysis revealed that samples lacking Ziziphora extract had a smooth surface, while those containing the extract displayed a rough texture that may potentially accelerate the wound healing process.