An improved pathway for autonomous bioluminescence imaging in eukaryotes.

Authors :: Shakhova ES
Karataeva TA
Markina NM
Mitiouchkina T
Palkina KA
Perfilov MM
Wood MG
Hoang TT
Hall MP
Fakhranurova LI
Alekberova AE
Malyshevskaia AK
Gorbachev DA
Bugaeva EN
Pletneva LK
Babenko VV
Boldyreva DI
Gorokhovatsky AY
Balakireva AV
Gao F
Choob VV
Encell LP
Wood KV
Yampolsky IV
Sarkisyan KS
Mishin AS
Source :: Nature methods [Nat Methods] 2024 Mar; Vol. 21 (3), pp. 406-410. Date of Electronic Publication: 2024 Jan 22.
Publication Year :: 2024
Abstract: The discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging.<br /> (© 2024. The Author(s).)