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An improved pathway for autonomous bioluminescence imaging in eukaryotes.
- Source :
-
Nature methods [Nat Methods] 2024 Mar; Vol. 21 (3), pp. 406-410. Date of Electronic Publication: 2024 Jan 22. - Publication Year :
- 2024
-
Abstract
- The discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging.<br /> (© 2024. The Author(s).)
- Subjects :
- Animals
Mammals
Eukaryota
Luminescence
Subjects
Details
- Language :
- English
- ISSN :
- 1548-7105
- Volume :
- 21
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Nature methods
- Publication Type :
- Academic Journal
- Accession number :
- 38253843
- Full Text :
- https://doi.org/10.1038/s41592-023-02152-y