Back to Search
Start Over
Adaptation of coenzyme stimulation assays for the nutritional assessment of vitamins B1, B2 and B6 using the Cobas Bio centrifugal analyser.
- Source :
-
Annals of clinical biochemistry [Ann Clin Biochem] 1987 Jan; Vol. 24 ( Pt 1), pp. 41-6. - Publication Year :
- 1987
-
Abstract
- Adaptation of coenzyme stimulation assays for the nutritional assessment of thiamine, riboflavin and pyridoxine on the Cobas Bio centrifugal analyser are described. Whole blood was collected into acid-citrate dextrose, which preserves the erythrocytes, prior to assay for several days. Washed erythrocytes stored at -70 degrees C and subsequently thawed, showed altered enzyme activities. The methods offer improved precision over existing procedures and take advantage of the high throughput capabilities of the instrumentation.
- Subjects :
- Anorexia Nervosa complications
Anorexia Nervosa enzymology
Centrifugation
Clinical Enzyme Tests
Erythrocytes enzymology
Flavin-Adenine Dinucleotide metabolism
Glutathione metabolism
Glutathione Reductase blood
Humans
Mathematics
NAD metabolism
Transketolase blood
Vitamin B Deficiency blood
Coenzymes blood
Pyridoxine blood
Riboflavin blood
Thiamine blood
Vitamin B Deficiency diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 0004-5632
- Volume :
- 24 ( Pt 1)
- Database :
- MEDLINE
- Journal :
- Annals of clinical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 3827183
- Full Text :
- https://doi.org/10.1177/000456328702400106