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Exploratory Study on Micronuclei and Metanuclear Abnormalities in Exfoliated Buccal Cells of COVID-19 Suspected Patients.
- Source :
-
Journal of cytology [J Cytol] 2024 Jan-Mar; Vol. 41 (1), pp. 28-33. Date of Electronic Publication: 2023 Dec 28. - Publication Year :
- 2024
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Abstract
- Context: SARS-CoV-2 virus causes COVID-19 by infecting nasal and oral cavities primarily by attaching its spike proteins to ACE 2 receptors expressed in epithelial cells.<br />Aim: This study was done to evaluate the micronucleated cell count, metanuclear abnormalities, and genotoxic factor in exfoliated buccal mucosal cell among the COVID-19 suspected patients.<br />Settings and Design: This cross-sectional study was conducted at AIIMS, Mangalagiri, between August and October 2022.<br />Methods: One hundred COVID-19 suspected patients were recruited for this study after obtaining informed and written consent; buccal smear was obtained and stained for papanicolaou test (PAP). The PAP-stained slides were analyzed for micronuclei (MN), pyknotic, karyolytic, and karyorrhexic cell count, respectively. Based on their reverse transcription-polymerase chain reaction (RT-PCR) report, the patients were grouped into COVID-19 positive and negative groups.<br />Statistical Analysis: The genotoxicity factor was calculated using the micronucleated cell count from both the groups using mean and standard deviation.<br />Results: The MN, micronucleated cell, pyknotic, karyolitic, and karyorrhexic cell count in COVID-19 positive patients were 24.12, 15.24, 3.08, 2.88 and 4.40, respectively, than COVID-19 negative patients 5.69, 8.17, 1.08, 1.00 and 2.43, respectively. The genotoxicity factor for SARS-CoV-2 was 2.68 which is a positive genotoxic effect on buccal mucosal cells.<br />Conclusion: SARS-CoV-2 increases the expression of micronucleated cells, pyknotic cells, karyolytic cells, and karyorhexic cells and concludes SARS-CoV-2 is having cytogenotoxic effect on the buccal mucosal cells. This can be used as a reliable marker in identifying the early carcinogenic effects of virus causing COVID-19.<br />Competing Interests: There are no conflicts of interest.<br /> (Copyright: © 2023 Journal of Cytology.)
Details
- Language :
- English
- ISSN :
- 0970-9371
- Volume :
- 41
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of cytology
- Publication Type :
- Academic Journal
- Accession number :
- 38282807
- Full Text :
- https://doi.org/10.4103/joc.joc_53_23