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The ABI4-RGL2 module serves as a double agent to mediate the antagonistic crosstalk between ABA and GA signals.
- Source :
-
The New phytologist [New Phytol] 2024 Mar; Vol. 241 (6), pp. 2464-2479. Date of Electronic Publication: 2024 Jan 29. - Publication Year :
- 2024
-
Abstract
- Abscisic acid (ABA) and gibberellins (GA) antagonistically mediate several biological processes, including seed germination, but the molecular mechanisms underlying ABA/GA antagonism need further investigation, particularly any role mediated by a transcription factors module. Here, we report that the DELLA protein RGL2, a repressor of GA signaling, specifically interacts with ABI4, an ABA signaling enhancer, to act as a transcription factor complex to mediate ABA/GA antagonism. The rgl2, abi3, abi4 and abi5 mutants rescue the non-germination phenotype of the ga1-t. Further, we demonstrate that RGL2 specifically interacts with ABI4 to form a heterodimer. RGL2 and ABI4 stabilize one another, and GA increases the ABI4-RGL2 module turnover, whereas ABA decreases it. At the transcriptional level, ABI4 enhances the RGL2 expression by directly binding to its promoter via the CCAC cis-element, and RGL2 significantly upregulates the transcriptional activation ability of ABI4 toward its target genes, including ABI5 and RGL2. Abscisic acid promotes whereas GA inhibits the ability of ABI4-RGL2 module to activate transcription, and ultimately ABA and GA antagonize each other. Genetic analysis demonstrated that both ABI4 and RGL2 are essential for the activity of this transcription factor module. These results suggest that the ABI4-RGL2 module mediates ABA/GA antagonism by functioning as a double agent.<br /> (© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.)
- Subjects :
- Abscisic Acid pharmacology
Abscisic Acid metabolism
Germination
Gene Expression Regulation, Plant
Transcription Factors genetics
Transcription Factors metabolism
Gibberellins pharmacology
Gibberellins metabolism
Seeds genetics
Arabidopsis metabolism
Arabidopsis Proteins genetics
Arabidopsis Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8137
- Volume :
- 241
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The New phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 38287207
- Full Text :
- https://doi.org/10.1111/nph.19533