Effects of physical form of β -lactoglobulin and calcium ingestion on GLP-1 secretion, gastric emptying and energy intake in humans: a randomised crossover trial.

The aim of this study was to assess whether adding Ca ²⁺  to aggregate or native forms of β -lactoglobulin alters gut hormone secretion, gastric emptying rates and energy intake in healthy men and women. Fifteen healthy adults (mean ± sd: 9M/6F, age: 24 ± 5 years) completed four trials in a randomised, double-blind, crossover design. Participants consumed test drinks consisting of 30 g of β -lactoglobulin in a native form with (NATIVE + MINERALS) and without (NATIVE) a Ca ²⁺ -rich mineral supplement and in an aggregated form both with (AGGREG + MINERALS) and without the mineral supplement (AGGREG). Arterialised blood was sampled for 120 min postprandially to determine gut hormone concentrations. Gastric emptying was determined using ¹³ C-acetate and ¹³ C-octanoate, and energy intake was assessed with an ad libitum meal at 120 min. A protein × mineral interaction effect was observed for total glucagon-like peptide-1 (GLP-1 _TOTAL ) incremental AUC (iAUC; P < 0·01), whereby MINERALS + AGGREG increased GLP-1 _TOTAL iAUC to a greater extent than AGGREG (1882 ± 603 v . 1550 ± 456 pmol·l ^-1 ·120 min, P < 0·01), but MINERALS + NATIVE did not meaningfully alter the GLP-1 iAUC compared with NATIVE (1669 ± 547 v . 1844 ± 550 pmol·l ^-1 ·120 min, P = 0·09). A protein × minerals interaction effect was also observed for gastric emptying half-life ( P < 0·01) whereby MINERALS + NATIVE increased gastric emptying half-life compared with NATIVE (83 ± 14 v . 71 ± 8 min, P < 0·01), whereas no meaningful differences were observed between MINERALS + AGGREG v . AGGREG ( P = 0·70). These did not result in any meaningful changes in energy intake (protein × minerals interaction, P = 0·06). These data suggest that the potential for Ca ²⁺ to stimulate GLP-1 secretion at moderate protein doses may depend on protein form. This study was registered at clinicaltrials.gov (NCT04659902).