Back to Search
Start Over
Increased expression of complement C3c, iC3b, and cells containing CD11b or CD14 in experimentally induced psoriatic lesion.
- Source :
-
Clinical and experimental immunology [Clin Exp Immunol] 2024 May 16; Vol. 216 (3), pp. 252-261. - Publication Year :
- 2024
-
Abstract
- Psoriasis is a chronic inflammatory skin disease with a characteristic isomorphic reaction, i.e. the Köbner reaction, induced by slight epidermal trauma. In this study, the tape-stripping technique was used to induce the development of Köbner reaction in 18 subjects with psoriasis. Eight subjects developed a positive reaction. To study the early cellular changes, skin biopsies were taken at the baseline and subsequent time points of 2 h, 1 d, 3 d, and 7 d for the immunostaining of complement C3c, iC3b, and cells expressing complement receptor 3 (CD11b/CD18; a receptor of iC3b) or CD14. The results show that the positive Köbner reaction is associated with rapid (2 h-1 d) and sustained (3-7 d) increase in the expression of epidermal C3c and iC3b and dermal C3c. In addition, there was a positive correlation between CD11b+ and CD14+ cells in baseline and 2 h-1 d biopsies with a subsequent increase in CD11b+ and CD14+ cells in 3-7 d biopsies in the Köbner-positive group. In the Köbner-negative group, only a transient increase in epidermal iC3b at 2 h-1 d, as well as rapid (2 h-1 d) and sustained increase (3-7 d) in dermal iC3b and CD14+ cells, was observed. In experiments with cultured monolayer keratinocytes, a slight cell damage already at 30 mJ/cm2 ultraviolet B irradiation led to increased expression of C3c, but not iC3b. Therefore, there are marked differences between Köbner groups in respect to the expression of C3c, iC3b, and cells expressing CD11b or CD14. Of note is the rapid and sustained increase in epidermal C3c and iC3b in the positive Köbner reaction.<br /> (© The Author(s) 2024. Published by Oxford University Press on behalf of the British Society for Immunology.)
- Subjects :
- Humans
Male
Female
Adult
Middle Aged
Skin pathology
Skin immunology
Skin metabolism
Skin radiation effects
Biopsy
Epidermis metabolism
Epidermis immunology
Epidermis pathology
Lipopolysaccharide Receptors metabolism
Psoriasis immunology
Psoriasis metabolism
CD11b Antigen metabolism
Complement C3b metabolism
Complement C3b immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2249
- Volume :
- 216
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Clinical and experimental immunology
- Publication Type :
- Academic Journal
- Accession number :
- 38310540
- Full Text :
- https://doi.org/10.1093/cei/uxae009