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IL-32 production from lung adenocarcinoma cells is potentially involved in immunosuppressive microenvironment.
- Source :
-
Medical molecular morphology [Med Mol Morphol] 2024 Jun; Vol. 57 (2), pp. 91-100. Date of Electronic Publication: 2024 Feb 06. - Publication Year :
- 2024
-
Abstract
- Interleukin 32 (IL-32) is a proinflammatory cytokine secreted from several kinds of cancer cells. In the present study, we investigated the significance of IL-32 in lung adenocarcinoma by immunohistochemistry and bioinformatics analysis. IL-32 was positive in cancer cells of 21 cases (9.2%) of total 228 cases. Increased IL-32 gene expression was linked to worse clinical course in TCGA analysis, however, IL-32 expression in immunohistochemistry was not associated to clinical course in our cohort. It was also found that high IL-32 expression was seen in cases with increased lymphocyte infiltration. In vitro studies indicated that IFN-γ induced gene expression of IL-32 and PD1-ligands in lung adenocarcinoma cell lines. IL-32, especially IL-32β, also induced overexpression of PD1-ligands in human monocyte-derived macrophages. Additionally, Cancer-cell-derived IL-32 was elevated by stimulation with anticancer agents. In conclusion, IL-32 potentially induced by inflammatory conditions and anticancer therapy and contribute to immune escape of cancer cells via development the immunosuppressive microenvironment. IL-32 might be a target molecule for anti-cancer therapy.<br /> (© 2024. The Author(s) under exclusive licence to The Japanese Society for Clinical Molecular Morphology.)
- Subjects :
- Humans
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Macrophages immunology
Macrophages metabolism
Interferon-gamma metabolism
Interferon-gamma genetics
Interferon-gamma immunology
Immunohistochemistry
Male
A549 Cells
Tumor Microenvironment immunology
Interleukins metabolism
Interleukins genetics
Adenocarcinoma of Lung immunology
Adenocarcinoma of Lung pathology
Adenocarcinoma of Lung genetics
Adenocarcinoma of Lung metabolism
Lung Neoplasms immunology
Lung Neoplasms pathology
Lung Neoplasms genetics
Lung Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1860-1499
- Volume :
- 57
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Medical molecular morphology
- Publication Type :
- Academic Journal
- Accession number :
- 38316697
- Full Text :
- https://doi.org/10.1007/s00795-023-00378-5