Back to Search Start Over

Equipping Saccharomyces cerevisiae with an Additional Redox Cofactor Allows F 420 -Dependent Bioconversions in Yeast.

Authors :
Lee M
Fraaije MW
Source :
ACS synthetic biology [ACS Synth Biol] 2024 Mar 15; Vol. 13 (3), pp. 921-929. Date of Electronic Publication: 2024 Feb 12.
Publication Year :
2024

Abstract

Industrial application of the natural deazaflavin cofactor F <subscript>420</subscript> has high potential for the enzymatic synthesis of high value compounds. It can offer an additional range of chemistry to the use of well-explored redox cofactors such as FAD and their respective enzymes. Its limited access through organisms that are rather difficult to grow has urged research on the heterologous production of F <subscript>420</subscript> using more industrially relevant microorganisms such as Escherichia coli . In this study, we demonstrate the possibility of producing this cofactor in a robust and widely used industrial organism, Saccharomyces cerevisiae , by the heterologous expression of the F <subscript>420</subscript> pathway. Through careful selection of involved enzymes and some optimization, we achieved an F <subscript>420</subscript> yield of ∼1.3 μmol/L, which is comparable to the yield of natural F <subscript>420</subscript> producers. Furthermore, we showed the potential use of F <subscript>420</subscript> -producing S. cerevisiae for F <subscript>420</subscript> -dependent bioconversions by carrying out the whole-cell conversion of tetracycline. As the first demonstration of F <subscript>420</subscript> synthesis and use for bioconversion in a eukaryotic organism, this study contributes to the development of versatile bioconversion platforms.

Details

Language :
English
ISSN :
2161-5063
Volume :
13
Issue :
3
Database :
MEDLINE
Journal :
ACS synthetic biology
Publication Type :
Academic Journal
Accession number :
38346396
Full Text :
https://doi.org/10.1021/acssynbio.3c00718