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Equipping Saccharomyces cerevisiae with an Additional Redox Cofactor Allows F 420 -Dependent Bioconversions in Yeast.
- Source :
-
ACS synthetic biology [ACS Synth Biol] 2024 Mar 15; Vol. 13 (3), pp. 921-929. Date of Electronic Publication: 2024 Feb 12. - Publication Year :
- 2024
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Abstract
- Industrial application of the natural deazaflavin cofactor F <subscript>420</subscript> has high potential for the enzymatic synthesis of high value compounds. It can offer an additional range of chemistry to the use of well-explored redox cofactors such as FAD and their respective enzymes. Its limited access through organisms that are rather difficult to grow has urged research on the heterologous production of F <subscript>420</subscript> using more industrially relevant microorganisms such as Escherichia coli . In this study, we demonstrate the possibility of producing this cofactor in a robust and widely used industrial organism, Saccharomyces cerevisiae , by the heterologous expression of the F <subscript>420</subscript> pathway. Through careful selection of involved enzymes and some optimization, we achieved an F <subscript>420</subscript> yield of ∼1.3 μmol/L, which is comparable to the yield of natural F <subscript>420</subscript> producers. Furthermore, we showed the potential use of F <subscript>420</subscript> -producing S. cerevisiae for F <subscript>420</subscript> -dependent bioconversions by carrying out the whole-cell conversion of tetracycline. As the first demonstration of F <subscript>420</subscript> synthesis and use for bioconversion in a eukaryotic organism, this study contributes to the development of versatile bioconversion platforms.
Details
- Language :
- English
- ISSN :
- 2161-5063
- Volume :
- 13
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- ACS synthetic biology
- Publication Type :
- Academic Journal
- Accession number :
- 38346396
- Full Text :
- https://doi.org/10.1021/acssynbio.3c00718