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The peptide GOLVEN10 alters root development and noduletaxis in Medicago truncatula.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2024 May; Vol. 118 (3), pp. 607-625. Date of Electronic Publication: 2024 Feb 15. - Publication Year :
- 2024
-
Abstract
- The conservation of GOLVEN (GLV)/ROOT MERISTEM GROWTH FACTOR (RGF) peptide encoding genes across plant genomes capable of forming roots or root-like structures underscores their potential significance in the terrestrial adaptation of plants. This study investigates the function and role of GOLVEN peptide-coding genes in Medicago truncatula. Five out of fifteen GLV/RGF genes were notably upregulated during nodule organogenesis and were differentially responsive to nitrogen deficiency and auxin treatment. Specifically, the expression of MtGLV9 and MtGLV10 at nodule initiation sites was contingent upon the NODULE INCEPTION transcription factor. Overexpression of these five nodule-induced GLV genes in hairy roots of M. truncatula and application of their synthetic peptide analogues led to a decrease in nodule count by 25-50%. Uniquely, the GOLVEN10 peptide altered the positioning of the first formed lateral root and nodule on the primary root axis, an observation we term 'noduletaxis'; this decreased the length of the lateral organ formation zone on roots. Histological section of roots treated with synthetic GOLVEN10 peptide revealed an increased cell number within the root cortical cell layers without a corresponding increase in cell length, leading to an elongation of the root likely introducing a spatiotemporal delay in organ formation. At the transcription level, the GOLVEN10 peptide suppressed expression of microtubule-related genes and exerted its effects by changing expression of a large subset of Auxin responsive genes. These findings advance our understanding of the molecular mechanisms by which GOLVEN peptides modulate root morphology, nodule ontogeny, and interactions with key transcriptional pathways.<br /> (© 2024 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)
- Subjects :
- Indoleacetic Acids metabolism
Indoleacetic Acids pharmacology
Plant Root Nodulation genetics
Meristem genetics
Meristem growth & development
Meristem drug effects
Peptides metabolism
Peptides genetics
Medicago truncatula genetics
Medicago truncatula growth & development
Medicago truncatula metabolism
Medicago truncatula drug effects
Medicago truncatula physiology
Plant Proteins genetics
Plant Proteins metabolism
Plant Roots growth & development
Plant Roots genetics
Plant Roots drug effects
Plant Roots metabolism
Gene Expression Regulation, Plant
Root Nodules, Plant genetics
Root Nodules, Plant growth & development
Root Nodules, Plant metabolism
Root Nodules, Plant drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1365-313X
- Volume :
- 118
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 38361340
- Full Text :
- https://doi.org/10.1111/tpj.16626