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Molecular characterization and toxigenic profiles of Bacillus cereus isolates from foodstuff and food poisoning outbreaks in Brazil.
- Source :
-
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Jun; Vol. 55 (2), pp. 1693-1701. Date of Electronic Publication: 2024 Mar 06. - Publication Year :
- 2024
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Abstract
- Bacillus cereus sensu stricto (s.s.) is a well-known foodborne pathogen that produces a range of enterotoxins and is able to cause two different types of foodborne illnesses-the emetic and the diarrheal syndromes. In this study, 54 B. cereus s.s. strains isolated from foodstuff and foods involved in food poisoning outbreaks were characterized according to the presence of toxin-encoding genes, virulence-encoding genes, and panC typing. Most isolates were assigned to panC groups IV (61.1%) and III (25.9%), but members of groups II and V could also be found. Investigation of specific alleles revealed high numbers of isolates carrying toxin and other virulence genes including nheA (100%), nheB (100%), hblA (79.6%), hblC (79.6%), hblD (74.1%), cytK-2 (61.1%), clo (100%), pc-plc (75.9%), sph (68.5%), pi-plc (66.6%), hlyIII (62.9%), and hlyII (24.1%). All isolates were negative for ces and cytK-1. In summary, we detected various enterotoxin and other virulence factor genes associated with diarrheal syndrome in strains analyzed, implicated or not with food poisoning. Furthermore, the most isolates analyzed belong to high-risk phylogenetic groups' panC types III and IV. Our study provides a convenient molecular scheme for characterization of B. cereus s.s. strains responsible for food poisoning outbreaks in order to improve the monitoring and investigation and assess emerging clusters and diversity of strains.<br /> (© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
- Subjects :
- Brazil epidemiology
Humans
Virulence Factors genetics
Gram-Positive Bacterial Infections microbiology
Gram-Positive Bacterial Infections epidemiology
Bacillus cereus genetics
Bacillus cereus isolation & purification
Bacillus cereus classification
Bacillus cereus pathogenicity
Foodborne Diseases microbiology
Foodborne Diseases epidemiology
Disease Outbreaks
Enterotoxins genetics
Food Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1678-4405
- Volume :
- 55
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
- Publication Type :
- Academic Journal
- Accession number :
- 38446406
- Full Text :
- https://doi.org/10.1007/s42770-024-01283-4