Host specific Eimeria genus diagnosis and qPCR development in Ovis aries and Capra hircus.

Authors :: Li S
Jian Y
Zhang K
Li X
Wang R
Zhang L
Jian F
Source :: Journal of microbiological methods [J Microbiol Methods] 2024 May; Vol. 220, pp. 106910. Date of Electronic Publication: 2024 Mar 06.
Publication Year :: 2024
Abstract: The objective of the present study was to develop a real-time PCR (qPCR) technique for the diagnosis of Eimeria spp. in Ovis aries and Capra hircus. The qPCR technique was developed using SYBR Green, resulting in a PCR with high sensitivity, specificity, and reproducibility.<br />Competing Interests: Declaration of competing interest None.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)

Subjects :: Animals
Reproducibility of Results
Goats
Real-Time Polymerase Chain Reaction
Sheep, Domestic
Eimeria

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